Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. What is glutathione and why must we protect patient access to it? Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Acetaminophen Induced Hepatotoxicity: a Comprehensive Update What to Avoid When Taking Glutathione (Must Read Guide) Proactive Healthcare Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome Scientific Reports